L-Selectin antibody

Catalog No. ABIN320345

The Mouse Monoclonal anti-L-Selectin antibody has been validated for FACS. It is suitable to detect L-Selectin in samples from Chemical.

Quick Overview for L-Selectin antibody (ABIN320345)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: CC32

Product Details anti-L-Selectin Antibody

Specificity: This antibody recognises the bovine L-Selectin molecule, also known as CD62L.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L / L-Selectin

Target Type: Chemical

Background: The Human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. Human CD62L (L Selectin) is comprised of an aminoterminal C type lectin binding domain, an epidermal growth factor like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells. It is expressed on bone marrow myeloid progenitor cells, erythroid precursor cells and some thymocytes. It is preferentially localized to the tips of lymphocytes and polymorphonuclear cells microvilli. Bioactive soluble L Selectin derived from lymphocytes and neutrophils is present in normal and pathological biological fluids.Synonyms: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL

Gene ID: 281485